As the industry expands its repertoire of antibody drug products into new therapeutic areas, product formats and protein constructs, the control of antibody/antigen targeting, binding and specificity will take on a new level of importance for researchers

CHI’s PepTalk: The Protein Science Week is more than just a conference.

By combining modular building blocks, fusion protein therapeutics have an advantage over antibody-based therapies resulting in a customizable functionality.

This digital course will aim to guide the researcher in designing studies for accelerated stability testing of biologics. The course will begin with basic underlying concepts governing protein drug product stability, and focus on design principles for meaning stress and accelerated stability testing of not only the protein of interest but also of excipients and primary packaging components. Strategies to handle complexities arising from their interactions will also be discussed.

Together with input from Sarah Bondos from Texas A&M University, Mario Lebendiker of the Wolfson Centre for Applied Structural Biology at The Hebrew University of Jerusalem provides an indepth overview of protein purification with a particular focus on aggregation problems. This course reviews: common causes of protein aggregation during expression and purification, methods to detect the presence of aggregates, the types and properties of chemicals that, when added to buffers, may improve protein solubility, and methods to screen these additives to rapidly optimize the solubility of your protein.

This course provides both an overview of non-mammalian expression systems, as well as an in-depth exploration of both Escherichia coli and different strains of yeast as expression platforms. Both Instructors, Dom Esposito of SAIC-Frederick/NIH and Piotr Bobrowicz of Adimab, possess many years of experience with their specific expression host. They share their insights and extensive knowledge to help those in the lab achieve optimized expression. This DVD is perfect for those who want to learn about using E.coli as an expression system, and for researchers wishing to build and expand their mastery.